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投稿时间：2025-04-11 网络发布日期：2026-03-04
投稿时间：2025-04-11 网络发布日期：2026-03-04
中文摘要: 目的 探讨胃癌组织中有丝分裂阻滞缺陷蛋白2同源蛋白1（MAD2L1）和微小RNA（miR）-28-5p的表达水平及其与胃癌患者临床病理特征的关系，以期为胃癌的早期诊断及治疗提供参考。方法 从基因表达综合数据库（GEO）下载胃癌差异表达基因，确定MAD2L1作为此次研究的靶基因。基LinkedOmics网站，筛选MAD2L1相关miR，利用Targetscan验证MAD2L1与miR-28-5p有无靶向关系。收集2024年5月至11月徐州医科大学附属医院94例胃癌患者手术切除的肿瘤组织，同时获取距离肿瘤边缘5 cm及以上的对应癌旁组织。通过免疫组化和逆转录定量聚合酶链式反应（RT-qPCR）分别检测MAD2L1和miR-28-5p的表达水平，采用Spearman相关系数分析二者的相关性；通过χ2检验分析MAD2L1和miR-28-5p的表达水平与胃癌患者临床病理特征之间的关系。结果 生物信息学分析显示，在胃癌中，MAD2L1高表达，miR-28-5p低表达，MAD2L1与miR-28-5p具有靶向关系。免疫组化结果显示，胃癌组织中MAD2L1的表达率（56.38%）明显高于癌旁组织（39.36%）（χ2=5.457，P<0.05）。RT-qPCR结果显示，胃癌中miR-28-5p的表达水平（0.26±0.08）明显低于癌旁组织（1.14±0.53）（t=16.048，P<0.01）。胃癌组织中MAD2L1表达与miR-28-5p表达呈负相关（r=-0.369，P<0.01）。胃癌组织中MAD2L1表达与临床分期、淋巴结转移、分化程度、神经侵犯及脉管侵犯有关（P<0.05），miR-28-5p 表达与分化程度有关（P<0.05）。结论 MAD2L1和miR-28-5p表达与胃癌患者临床病理特征密切相关，两者可作为临床上胃癌诊疗中的潜在生物标志物。
中文关键词: 胃癌 有丝分裂阻滞缺陷蛋白2同源蛋白1 miR-28-5p 基因表达综合数据库 临床病理特征
Abstract:Objective To explore the expression levels of mitotic arrest deficient 2 like 1（MAD2L1）and microRNA（miR）-28-5p in gastric cancer tissues and their relationship with the clinicopathological characteristics of gastric cancerpatients，in order to provide a reference for early diagnosis and treatment. Methods The differentially expressed genes in gastric cancer were downloaded from the Gene Expression Omnibus（GEO）database，and MAD2L1 was identified as the target gene for this study. Based on the LinkedOmics website，the miRs related to MAD2L1 were screened，and whether there was a targeting relationship between MAD2L1 and miR-28-5p was verified using Targetscan. Tumor tissues surgically resected from 94 gastric cancer patients at the Affiliated Hospital of Xuzhou Medical University were collected from May to November 2024，along with corresponding adjacent tissues obtained at least 5 cm away from the tumor margin. The expression levels of MAD2L1 and miR-28-5p were detected by immunohistochemistry and reverse transcription quantitative polymerase chain reaction（RT-qPCR），respectively. The correlation between the two was analyzed by the Spearman correlation coefficient，and their relationship with the clinicopathological characteristics of gastric cancer patients was analyzed by the chi-squared test. Results Bioinformatics analysis revealed that MAD2L1 was upregulated and miR-28-5p was downregulated in gastric cancer. MAD2L1 had a targeting relationship with miR-28-5p. The immunohistochemical results showed that the expression rate of MAD2L1 in gastric cancer tissues（56.38%）was significantly higher than that in adjacent tissues（39.36%）（χ2=5.457，P < 0.05）. The RT-qPCR results showed that the expression level of miR-28-5p in gastric cancer（0.26 ± 0.08）was significantly lower than that in adjacent tissues（1.14±0.53）（t=16.048，P<0.01）. The expression of MAD2L1 was negatively correlated with the expression of miR-28-5p in gastric cancer tissues（r=-0.369，P<0.01）. The expression of MAD2L1 in gastric cancer tissues was associated with the clinical stage，lymph node metastasis，degree of differentiation，nerve invasion and vascular invasion（P<0.05），and the expression of miR-28-5p was associated with the degree of differentiation（P<0.05）. Conclusion The expression of MAD2L1 and miR-28-5p is closely related to the clinicopathological characteristics of gastric cancer patients，and both can be used as potential biomarkers in the diagnosis and treatment of gastric cancer in clinical practice.
keywords: Gastric cancer Mitotic arrest deficient 2 like 1 microRNA-28-5p Gene Expression Omnibus database Clinicopathological characteristics
文章编号： 中图分类号：R735 文献标志码：A
基金项目:
附件
| 作者 | 单位 |
| 崔广洁 | 1. 徐州医科大学研究生院, 江苏 徐州 221000 |
| 刘袁明 | 1. 徐州医科大学研究生院, 江苏 徐州 221000 |
| 陈佳琪 | 1. 徐州医科大学研究生院, 江苏 徐州 221000 |
| 陆杨 | 1. 徐州医科大学研究生院, 江苏 徐州 221000 |
| 陶云霞 | 2. 徐州医科大学附属医院肿瘤科, 江苏 徐州 221000 |
| 高超 | 2. 徐州医科大学附属医院肿瘤科, 江苏 徐州 221000 |
| Author Name | Affiliation |
| CUI Guangjie*，LIU Yuanming，CHEN Jiaqi，LU Yang，TAO Yunxia，GAO Chao | *Graduate School of Xuzhou Medical University,Xuzhou,Jiangsu 221000,China |
引用文本：
崔广洁,刘袁明,陈佳琪,等.MAD2L1和miR-28-5p在胃癌组织中的表达及临床意义[J].中国临床研究,2026,39(2):193-198.
崔广洁,刘袁明,陈佳琪,等.MAD2L1和miR-28-5p在胃癌组织中的表达及临床意义[J].中国临床研究,2026,39(2):193-198.