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投稿时间：2025-04-16 网络发布日期：2026-03-04
投稿时间：2025-04-16 网络发布日期：2026-03-04
中文摘要: 目的 研究异戊二烯羧基甲基转移酶（ICMT）在胃癌中的表达水平及对疾病进程的影响。方法 回顾性收集兰州大学第二医院2022年5月至9月确诊为胃癌并行手术治疗的60例患者的临床资料及手术标本，通过免疫组织化学法（IHC）及聚合酶链式反应（PCR）技术，对比癌组织和癌旁组织中ICMT mRNA和蛋白的表达水平，并分析ICMT表达水平与患者临床特征之间的关联性。结果 IHC结果显示，癌组织结构紊乱、细胞排列杂乱、极向丧失，胞浆中ICMT蛋白的阳性颗粒数显著高于癌旁正常组织。PCR结果显示，ICMT mRNA在胃癌组织的表达水平显著高于癌旁组织（P<0.05）。与临床特征的关联性分析显示，胃癌患者中高表达ICMT mRNA的占比均呈现由高到低的分布趋势，且差异具有统计学意义：（1）病变部位，胃体癌>贲门癌>胃窦癌（χ2=7.161，P=0.028）；（2）Lauren分型，混合型>肠型>弥漫型（χ2=13.153，P=0.001）；（3）分化程度，低分化>中高分化（χ2=7.625，P=0.006）；（4）T分期，T3+T4期>T1+T2期（χ2=5.740，P=0.017）；（5）p53蛋白表达（基于病理结论>），突变型>野生型（χ2=5.831，P=0.016）。结论 ICMT 在胃癌患者中高表达，且表达差异与胃癌的病变部位、分化程度、病程阶段存在关联，p53基因突变可能是胃癌组织ICMT 高表达的病理基础。
中文关键词: 异戊二烯羧基甲基转移酶 大鼠肉瘤蛋白 胃癌 p53基因 靶点
Abstract:Objective To study the expression level of isoprenyl carboxyl methyltransferase（ICMT）in gastric cancer and its effect on disease progression. Methods A clinical retrospective study was conducted on 60 patients diagnosed with gastric cancer who underwent surgical treatment at Lanzhou University Second Hospital between May and September 2022. The expression levels of ICMT mRNA and protein in tumor tissue and adjacent tissue were compared using immunohistochemistry（IHC） and polymerase chain reaction （PCR） techniques. The correlation between ICMT expression levels and patient clinical characteristics was analyzed. Results IHC showed structural disorganization of tumor tissue，scattered cell arrangement，and loss of polarity. The number of positive ICMT protein granules in gastric tumor tissue was significantly higher than that in adjacent tissues. PCR showed that the expression levels of ICMT mRNA in gastric tumor tissue were significantly higher than those in adjacent tissue（P<0.05）. Correlation analysis with clinical features showed that the proportion of gastric cancer patients having high expression of ICMT mRNA presented a distribution trend from high to low in the following clinical characteristics，with statistically significant differences.（1）Cancer locations：gastric corpus cancer > cardiac cancer > gastric antrum cancer（χ2＝7.161，P=0.028）；（2）Lauren’s classification：mixed > intestinal > diffuse（χ2=13.153，P=0.001）；（3）grades of differentiation：poorly differentiated >moderately to well differentiated（χ2=7.625，P=0.006）；（4）T stage：（T3 +T4）stage >（T1 +T2）stage（χ2= 5.740，P=0.017）；（5）p53 protein expression（based on the conclusions of the patients?? pathology reports）：mutated type > wild type（χ2=5.831，P=0.016）. Conclusion ICMT is highly expressed in gastric cancer，and the difference in expression isassociated with the location of the lesion，degree of differentiation，and stage of gastric cancer. p53 mutation may be the basis for the high expression of ICMT in gastric tumor tissue.
文章编号： 中图分类号：R735.2 文献标志码：A
基金项目:甘肃省自然科学基金资助项目（22JR5RA958）；兰州大学第二医院“萃英科技创新”计划资助项目（CY2021-QN-B07）；2022年萃英学子科研培育计划（CYXZ2022-20）
附件
| Author Name | Affiliation |
| JING Tiancong*，MA Shangxian，LI Yuanxiao，DAI Xingxing，NIU Shaomin，MA Hanwei | *Second Clinical Medical College of Lanzhou University,Lanzhou,Gansu 730030,China |
引用文本：
景天聪,马尚贤,李元枭,等.异戊二烯羧基甲基转移酶在胃癌组织中的表达与临床意义[J].中国临床研究,2026,39(2):188-192.
景天聪,马尚贤,李元枭,等.异戊二烯羧基甲基转移酶在胃癌组织中的表达与临床意义[J].中国临床研究,2026,39(2):188-192.