Abstract:Glioblastoma（GBM）is one of the most common primary brain tumors in adults，characterized by rapid progression and extremely poor prognosis. Cholesterol metabolism reprogramming occurs in GBM cells，involving the processes of cholesterol uptake，transport and esterification. Tumor cells can use cholesterol as an energy source，construct cell membranes and mediate signal molecules to promote their proliferation，progression and adaptation to the tumor microenvironment. Recent studies have shown that cholesterol metabolism in GBM cells is closely related to patient prognosis，and therapies targeting its regulatory pathways can significantly improve survival time. This review summarizes the phenomenon of cholesterol metabolism reprogramming in GBM，the roles of sterol regulatory element-binding proteins，liver X receptors，3-hydroxy-3-methylglutaryl coenzyme A reductase（HMGCR）and others in the regulation of cholesterol metabolism，and interpret the latest advances in targeted therapies related to cholesterol metabolism and its regulatory pathways.