Abstract:Objective To analyze the diagnosis value of high mobility group box 1 protein（HMGB1) combined with T helper cell 17(Th17) in Alzheimer′s disease(AD),and to provides evidence for clinical application.MethodFrom July 2019 to February 2020,107 patients from the Second Affiliated Hospital of Shanxi Medical University with AD were selected as the AD group and 107 healthy people were collected as the control group.Serum HMGB1 and Th17 levels were detected by enzyme-linked immunosorbent assay and flow cytometry.ROC curve was used to analyze the efficacy of HMGB1 and Th17 alone and in combination in diagnosing AD.Results HMGB1 and Th17 in AD group were higher than those in control group,the difference was statistically significant ( P<0.01).The serum levels of HMGB1 and Th17 in AD patients with severe consciousness disorder were higher than those in patients with mild consciousness disorder ( P<0.01).The specificity and sensitivity of combined testing in the diagnosis of AD were higher than those of HMGB1 alone and Th17 alone.The area under the ROC curve (AUC) of HMGB1 and Th17 for AD diagnosis accuracy were 0.754 and 0.812,respectively.Conclusions HMGB1 and Th17 are highly sensitive and specific markers of AD,which are helpful for monitoring the condition of AD patients,observing the efficacy and guiding treatment.The combined use of HMGB1 and Th17 is of great significance for the differential diagnosis of AD.