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Received:April 10, 2020 Published Online:November 20, 2020
Received:April 10, 2020 Published Online:November 20, 2020
中文摘要: 目的 探讨桃仁红花煎对2型糖尿病模型大鼠血糖、血脂代谢及肾损伤的影响。方法 将100只健康清洁级SD大鼠随机分为4组：健康对照组(正常喂养,不做药物处理)，模型组(采用STZ注射法建立糖尿病模型)，二甲双胍干预组(建立糖尿病大鼠模型后给予二甲双胍灌胃处理)和桃仁红花煎干预组(建立糖尿病大鼠模型后给予桃仁红花煎灌胃处理)。给药30 d后,记录各组大鼠体重,留取24 h尿检测尿蛋白量,检测各组大鼠空腹血糖(FPG)、空腹血清胰岛素(FINS)及血清胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及血肌酐(Scr)、血尿素氮(BUN)含量,计算肾脏指数、胰岛素抵抗指数(IRI),苏木素-伊红染色(HE)观察大鼠肾脏病理变化,检测各组大鼠肾脏组织中Wnt-1、β-catenin mRNA和蛋白表达水平。结果 与健康对照组比较,模型组大鼠FPG及FINS水平升高(P<0.05),IRI和血清TC、TG、LDL-C含量升高(P<0.05),血清HDL-C含量降低(P<0.05)；肾组织病理观察显示大鼠肾小球肿胀、体积增大,基底膜增厚且系膜基质增多,肾小囊变窄,大鼠肾脏指数增加(P<0.05)；尿蛋白及血Scr、BUN升高(P<0.05),肾脏组织中Wnt-1、β-catenin mRNA和蛋白相对表达水平升高(P<0.05)。与模型组比较,二甲双胍干预组和桃仁红花煎干预组大鼠FPG和血清FINS水平降低(P<0.05),IRI及血清TC、TG、LDL-C含量降低(P<0.05),血清HDL-C含量升高（P<0.05）；肾组织病理观察显示大鼠肾小球和肾小囊病变有所恢复,大鼠肾脏指数减小(P<0.05)；尿蛋白及血Scr、BUN降低(P<0.05),肾脏组织中Wnt-1、β-catenin mRNA和蛋白相对表达水平降低(P<0.05)。结论 桃仁红花煎能够有效降低糖尿病大鼠血糖、血脂水平,抑制肾脏Wnt/β-catenin信号通路,对大鼠肾脏损伤具有改善作用。
Abstract:Objective To investigate the influence of Taoren Honghua decoction on blood glucose,lipid metabolism and renal injury in rats with type 2 diabetes mellitus.Methods A total of 100 healthy SD rats were divided into 4 groups randomly ( n=25,each):healthy control group (normal feeding,without drug treatment),model group (streptozotocin injection modeling method was used to establish diabetic rats),metformin intervention group (streptozotocin injection + metformin intragastric administration) and Taoren Honghua decoction intervention group (streptozotocin injection+ Taoren Honghua decoction intragastric administration).At the end of administration,the body weight of rats in each group was recorded,24 h urine was taken for urine protein detection,fasting blood glucose (FPG),fasting serum insulin (FINS),serum cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),blood creatinine (Scr) and urea nitrogen (BUN) were measured.Renal index and insulin resistance index (IRI) were calculated.Hematoxylin eosin (HE) staining was used to observe the pathological changes of kidney.The mRNA and protein expressions of Wnt-1 and β-catenin in renal tissues were detected.Results (1) Compared with the healthy control group,the levels of FPG and FINS were increased ( P<0.05),IRI and serum TC,TG,LDL-C contents were increased ( P<0.05),serum HDL-C content was decreased ( P<0.05)in model group.Renal histopathological observation showed that glomerular swelling,volume increased,basement membrane thickening and mesangial matrix increased,renal capsule narrowed,kidney index increased ( P<0.05).The expression of Wnt-1,β-catenin mRNA and protein in kidney tissue increased ( P<0.05).(2) Compared with the model group,the levels of FPG and serum FINS in the metformin intervention group and Taoren Honghua decoction intervention group were decreased ( P<0.05),IRI and serum TC,TG,LDL-C content were decreased ( P<0.05),serum HDL-C content was increased( P<0.05);glomerular and renal capsule lesions were recovered,renal index was decreased ( P<0.05);the urine protein,blood Scr and BUN decreased ( P<0.05),and the relative expression levels of Wnt-1,β-catenin mRNA and protein in kidney tissue were decreased ( P<0.05).Conclusions Taoren Honghua decoction could effectively reduce the levels of blood glucose and blood lipid in diabetic rats,inhibit the Wnt/β-catenin signaling pathway in kidney,and improve renal injury in rats.
keywords: Type 2 diabetes mellitus Taoren Honghua decoction Rat Blood glucose Blood lipid Renal injury Insulin resistance index Kidney index
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基金项目:攀枝花市2016年度市级第一批科技计划项目(2016CY-k-5-2)
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