本文已被：浏览 853次 下载 521次
Received:August 20, 2019 Published Online:March 20, 2020
Received:August 20, 2019 Published Online:March 20, 2020
中文摘要: 目的 探讨热休克蛋白90(HSP90)和Polo样激酶1蛋白(PLK1)在脑胶质瘤的表达水平及与其病理分级的相关性。方法 对2014年1月至2018年12月临沂市人民医院康复科145例脑胶质瘤患者手术切除的不同病理级别的胶质瘤组织标本和10例非肿瘤患者正常脑组织标本进行HE染色检测;运用免疫组织化学方法检测HSP90、PLK1、Ki-67蛋白的表达情况,分析HSP90、PLK1与胶质瘤病理分级的相关性。结果 HSP90、PLK1、Ki-67在胶质瘤中的表达水平均高于正常脑组织(P<0.05)。Ki-67在正常脑组织中无表达,但在Ⅰ/Ⅱ、Ⅲ和Ⅳ分级胶质瘤组织中阳性表达率分别是39.13%、69.39%、86.00%,各级别间差异有统计学意义(P<0.05)。HSP90在正常脑组织中2例为弱阳性表达,在Ⅰ/Ⅱ、Ⅲ和Ⅳ分级胶质瘤组织中高表达率分别为36.96%、69.39%、50.00%,各级别间差异有统计学意义(P<0.05)。PLK1在正常脑组织中阴性表达,在Ⅰ/Ⅱ、Ⅲ和Ⅳ分级胶质瘤组织中高表达率分别为47.83%、83.67%、90.00%,各级别间差异有统计学意义(P<0.05)。结论 HSP90、PLK1蛋白在胶质瘤中的表达水平与其病理分级呈正相关,与疾病预后密切相关。
中文关键词: 热休克蛋白90 Polo样激酶1蛋白 Ki-67 胶质瘤 病理级别
Abstract:Objective To investigate the expression of heat shock protein 90 (HSP90) and Polo-like kinase 1 (PLK1) in gliomas and their correlation with pathological grade. Methods From January 2014 to December 2018, glioma tissue samples of different pathological grades from 145 patients with glioma and 10 normal brain tissues from non-tumor patients were collected, and all the tissues were detected by HE staining. The expression of HSP90, PLK1 and Ki-67 were detected by immunohistochemistry, and the correlation between HSP90, PLK1 and pathological grade of glioma was analyzed. Results The expression levels of HSP90, PLK1 and Ki-67 in glioma were significantly higher than that in normal brain tissue (all P<0.05). Ki-67 was not expressed in normal brain tissues, but the positive expression rates in grade Ⅰ/Ⅱ, Ⅲ and Ⅳ of gliomas were 39.13%, 69.39% and 86.00%, respectively (P<0.05). HSP90 was weakly positively expressed in 2 cases of normal brain tissues. The high expression rates in grade Ⅰ/Ⅱ, Ⅲ and Ⅳ of gliomas were 36.96%, 69.39% and 50.00%, respectively (P<0.05). PLK1 was negatively expressed in normal brain tissues, and its high expression rates in grade Ⅰ/Ⅱ, Ⅲ and Ⅳ of gliomas were 47.83%, 83.67% and 90.00%, respectively (P<0.05). Conclusion The expression levels of HSP90 and PLK1 protein in gliomas ware positively correlated with their pathological grades and are closely related to the prognosis of gliomas.
文章编号： 中图分类号： 文献标志码：A
基金项目:山东省自然科学基金(ZR2016HL16);临沂市科技创新发展计划项目(201919023)
引用文本：