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投稿时间：2025-04-03 网络发布日期：2025-09-19
投稿时间：2025-04-03 网络发布日期：2025-09-19
中文摘要: 目的 探讨Zeste同源蛋白增强子2（EZH2）和性别决定区域Y盒蛋白6（SOX6）在糖尿病肾病患者血清中的水平及与病情严重程度和肾功能的相关性。方法 选择2021年12月至2023年12月自贡市第一人民医院收治的120例糖尿病肾病患者作为回顾性研究的观察对象（糖尿病肾病组），纳入同期同院收治的120例单纯糖尿病患者作为糖尿病组。收集患者的临床资料，采用酶联免疫吸附试验检测血清EZH2和SOX6水平，全自动生化分析仪测定患者24h尿蛋白定量（24hUpro）、血清肌酐（Scr）、胱抑素-C（Cys-C）、血尿素氮（BUN）、尿白蛋白/尿肌酐比值（UACR）水平。根据估算肾小球滤过率（eGFR）将糖尿病肾病患者分为轻度组［60~＜90mL/（min· 1.73 m2 ），n=47］、中度组［30~＜60 mL/（min· 1.73 m2 ），n=40］、重度组［＜30 mL/（min· 1.73 m2 ），n=33］。采用多因素logistic回归分析糖尿病肾病患者病情严重程度的影响因素，采用Spearman与Pearson相关性分析血清EZH2、SOX6水平与糖尿病肾病患者病情严重程度、肾功能指标的相关性。结果 与糖尿病组比较，糖尿病肾病组患者血清EZH2［（26.52±5.25）pg/mL vs（4.13±0.62）pg/mL，t= 46.396，P＜0.01］、SOX6［（16.44±3.18）pg/mL vs（7.19±1.24）pg/mL，t= 29.687，P＜0.01］水平均较高。随着糖尿病肾病患者疾病严重程度的提高（轻度组→中度组→重度组），BUN、Cys- C、Scr、24 h Upro、EZH2、SOX6、UACR水平升高（P＜0.05）。多因素logistic回归分析显示高水平的BUN、Cys-C、Scr、24 h Upro、EZH2、SOX6、UACR是糖尿病肾病患者病情严重程度的危险因素（P＜0.05）。糖尿病肾病患者血清EZH2、SOX6水平分别与病情严重程度以及肾功能指标（BUN、Cys-C、Scr、24hUpro）水平呈正相关（P＜0.05）。结论 糖尿病肾病患者血清EZH2、SOX6水平均升高，二者均与糖尿病肾病进展及肾功能减退相关。
中文关键词: Zeste同源蛋白增强子2 性别决定区域Y盒蛋白6 糖尿病肾病 肾功能 肾小球滤过率
Abstract:Objective To investigate the serum levels of enhancer of zeste homolog 2 (EZH2) and sex-determining region Y box protein 6 (SOX6) in diabetic kidney disease (DKD) patients and their correlation with disease servity and renal function. Methods A retrospective study was conducted on 120 patients with DKD admitted to Zigong First People’ s Hospital from December 2021 to December 2023 (DKD group), and 120 patients with simple diabetes were included as the diabetic group. Clinical data were collected, and enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of EZH2 and SOX6. A fully automated biochemical analyzer was used to determine the 24- hour urine protein quantity (24 h Upro), serum creatinine (Scr), cystatin-C (Cys-C), and blood urea nitrogen (BUN), urine albumin - to - creatinine ratio (UACR) levels. According to the estimated glomerular filtration rate (eGFR), patients in the DKD group were divided into mild group [60-<90 mL/ (min· 1.73 m2 ), n=47], moderate group [30-<60 mL/ (min· 1.73 m2 ), n=40], and severe group [< 30 mL/ (min· 1.73 m2 ), n=33]. Multivariate logistic regression analysis was performed to identify the influencing factors of disease severity in DKD patients. Spearman and Pearson correlation analyses were used to evaluate the correlation of serum EZH2, SOX6 levels with disease severity and renal function indicators. Results Compared with the diabetic group, the DKD group had significantly higher serum levels ofEZH2 [ (26.52±5.25) pg/mL vs (4.13±0.62) pg/mL, t= 46.396, P＜0.01]and SOX6 [ (16.44±3.18) pg/mL vs (7.19±1.24) pg/mL, t= 29.687, P＜0.01]. As the severity of DKD increased (mild group→ moderate group→ severe group), levels of BUN, Cys-C, Scr, 24 h Upro, EZH2, SOX6, and UACR increased (P＜0.05). Multivariate logistic regression analysis showed that high levels of BUN, Cys-C, Scr, 24 h Upro, EZH2, SOX6, and UACR were risk factors for the severity of DKD (P＜0.05). In patients with DKD, serum EZH2 and SOX6 levels were positively correlated with disease severity and renal function indicators (BUN, Cys-C, Scr, 24 h Upro), respectively (P＜0.05). Conclusion The serum levels of EZH2 and SOX6 are elevated in DKD patients, and both are respectively associated with the progression of DKD and renal dysfunction.
keywords: Enhancer of zeste homolog 2 Sex-determining region Y box protein 6 Diabetic kidney disease Renal
文章编号： 中图分类号：R587.2 文献标志码：A
基金项目:
附件
| 作者 | 单位 |
| 李治成 | 自贡市第一人民医院肾病内科,四川 自贡 643000 |
| 郝炎 | 自贡市第一人民医院肾病内科,四川 自贡 643000 |
| 郑巧 | 自贡市第一人民医院肾病内科,四川 自贡 643000 |
| 李培莉 | 自贡市第一人民医院肾病内科,四川 自贡 643000 |
引用文本：
李治成,郝炎,郑巧,等.EZH2和SOX6在糖尿病肾病患者中的表达及与肾功能的相关性研究[J].中国临床研究,2025,38(9):1360-1363,1382.
李治成,郝炎,郑巧,等.EZH2和SOX6在糖尿病肾病患者中的表达及与肾功能的相关性研究[J].中国临床研究,2025,38(9):1360-1363,1382.