Abstract:Objective To investigate the therapeutic effect of platelet-rich plasma(PRP) on bone healing of nasal bone defect in rabbit model,and to provide experimental reference for clinical application. Methods Twenty-four adult New Zealand white rabbits weighting 2.5-3.0 kg,all male,were used to prepare nasal bone cylindrical defects with a diameter of 5 mm and a depth of 2.5 mm,and were randomly divided into PRP group (group A,n=12) and blank group (group B,n=12).The bone defect area was not treated with drugs in group B,while the bone defect area was filled with rabbit autologous PRP in group A.Gross observation,histological staining (HE staining,Masson staining）and CT value,type Ⅰ collagen in bone tissue(by immunofluorescence staining) and serum bone alkaline phosphatase (BALP,by ELISA method) levels were performed at 4,8 and 12 weeks after operation. Results The rabbit model of nasal bone defect was made successfully,and there was no infection or death in two groups after operation.The histological observation of newborn bone tissue showed that the number of fibrous tissue,blood vessels and osteoblasts in group A were significantly more than those in group B,and the positive staining intensity of type Ⅰ collagen was significantly stronger than that in group B.At 4,8 and 12 weeks in group A,the CT value of bone defect,the measured value of typeⅠ collagen in newborn bone tissue and the level of serum BALP were significantly higher than those in group B (P<0.01). Conclusion PRP has a strong promoting effect on bone healing of nasal bone defect in rabbit model.